10-Hydroxy-2-Decenoic Acid Prevents Ultraviolet A-Induced Expression of Lamin AΔ150 in Human Dermal Fibroblasts
10-Hydroxy-2-decenoic acid (10-HDA) as the main component of royal jelly has pharmacological characteristics. But the influence of 10-HDA on skin photoaging and photo damage is poorly understood. In the present study, we used 10-HAD immediately after UVA exposure and tested the effects on the attenuation of LMNAΔ150 expression in cultured human dermal fibroblasts.
Human dermal fibroblasts (cultured cells) were exposed to UVA irradiation. The mRNA level of LMNAΔ150 was determined by Taqman Real-Time PCR Assay.
Real-time PCR analysis of LMNAΔ150 transcripts indicated that the level of LMNAΔ150 transcripts was higher in the UVA exposed group than the group treated with 10-HAD after UVA exposure (>8.22-fold). The LMNAΔ150 expression is down-regulated in human dermal fibroblasts after treatment with 10-HDA.
It can be concluded that treatment with 10-HDA suppresses the UVA-induced gene expression of LMNAΔ150 and protects skin from UVA-induced photoaging and photo damage.
Keywords: 10-HAD, UVA, LMNAΔ150, photoaging.