Oana Maria PATRASCU

Latest posts by Oana Maria PATRASCU (see all)

Expression of Bcl-2, Melan A and HMB-45 in Dysplastic Nevi - March 31, 2016
Ciliary Body Melanoma – A Particularly Rare Type of Ocular Tumor. Case Report and General Considerations - July 2, 2015
Disseminated CMV and Tuberculosis Infection with Osseous Metaplasia in a Presumable Crohn’s Patient: Case Report - June 30, 2015

Articles signed on MÆDICA, JCM:

Expression of Bcl-2, Melan A and HMB-45 in Dysplastic Nevi

MÆDICA - a Journal of Clinical Medicine | Vol. 11, nr. 1, 2016

Oana Maria PATRASCU, Mariana COSTACHE, Adrian Vasile DUMITRU, Corina Nicoleta MEHOTIN, Maria SAJIN and Anca Mihaela LAZAROIU

ABSTRACT

Background: From the first recognition of dysplastic nevi as a pathology per se, many debates have been raised and many histological and immunohistological studies have been conducted in order to establish the true significance of these lesions. Therefore, the aim of this study was to establish if there is a correlation between HMB-45, Melan A and Bcl-2 expression and the grade of dysplasia, as well as between the marker’s staining patterns.

Material and Methods: Ten dysplastic nevi from six female patients were selected and their histological features (size, dysplasia), as well as the immunohistological staining patterns, were studied (HMB-45, Melan A, Bcl-2). The Pearson correlation coefficient and regression was calculated with Windows Excel Data Analysis.

Results: We demonstrated that there was a notable correlation between the dysplasia and the size of the lesions (r(8)= 0.62 with p-value= 0.052), and also between Melan A and Bcl-2 (a r(6)= 0.73, p<0.05), but we did not obtain a statistically significant correlation between other features (p>0.05).

Conclusions: We can affirm, at least in our cases, there is a correlation between the grade of dysplasia and the size of the lesion, and also, that there is a correlation between Melan A and Bcl-2 staining, explained by MITF gene. These results were only partial concordant with those in other studies, therefore a larger number of cases is recommended to be further analyzed in order to clearly draw a conclusion.

Keywords: dysplastic nevi, Bcl-2, Melan A, HMB-45, imunohistological stains

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Ciliary Body Melanoma – A Particularly Rare Type of Ocular Tumor. Case Report and General Considerations

Mariana COSTACHE, Oana Maria PATRASCU, Dumitru ADRIAN, Diana COSTACHE, Maria SAJIN, Emil UNGUREANU and Olga SIMIONESCU

ABSTRACT

Uveal melanoma is the most common primary malignancy of the eye in white adults. Frequently, uveal melanoma arises from choroid or iris. Ciliary body melanoma is a rare if not exceptional subtype of uveal melanoma. Furthermore, ciliary melanoma is often seen in association with the other two subtypes of uveal melanoma. This paper presents a case of primary ciliary melanoma with invasion of the iris. The patient presented with blurred vision, but this symptom could not doubtless be related with the existence of the tumor, because of the small dimension of the malignancy and the lack of medical history of the patient. This tumor was included in the category of “very small ciliary melanoma”, a rare diagnosis considering the fast local invasion and the lack of symptoms in such a small tumor. Histopathological and imunohistochemical examinations confirmed the diagnosis of ciliary melanoma, This type of ocular melanoma has a low prognosis due to early metastases.

Keywords: ciliary body, uveal melanoma, genetic predisposition, unfavorable prognosis

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Disseminated CMV and Tuberculosis Infection with Osseous Metaplasia in a Presumable Crohn’s Patient: Case Report

Oana Maria PATRASCU, Monica CIRSTOIU, Anca Mihaela LAZAROIU, Adrian Vasile DUMITRU, Andreea Elena MIHAI, Manuela POPA, Anca DIMITRIU, Maria SAJIN, Mariana COSTACHE and Andreea ILIESIU

ABSTRACT

Associations of different pathologies are not uncommon in every day practice, but association of disseminated infections like cytomegalovirus infection and tuberculosis are quite rare and hard to diagnose. Both are infections which appear frequently in immunocompromised patients and have unfavorable prognosis. We present a case of a 62 year old male with a history of Crohn’s disease and tuberculosis which presented with symptoms of relapse and infection. He was treated with immunosuppressive medication and cortisol for the past 6 weeks. Cytomegalovirus (CMV) infection was serologically confirmed. In evolution, he suffered from gastrointestinal hemorrhage and died afterwards due to the hemorrhage and pulmonary infections. Histology confirmed the CMV modification in the lungs and intestines, but also highlighted active and disseminated tuberculosis (TB), bronchopneumonia, osseous metaplasia, hyaline membranes, numerous TB abscesses in the intestinal wall and specific CMV and TB modifications in the liver. The trigger for such important and serious infections remains unclear, for the cause can be represented by the Crohn’s disease per se or only by the immunosuppressive treatment. Also, CMV can trigger modifications in immune system and patients with immune-mediated diseases have an increased risk for TB reactivation.

Keywords: cytomegalovirus, tuberculosis, Crohn’s disease, osseous metaplasia, gastro-intestinal hemorrhage

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A Comparison Between Clinical Diagnosis of Death and Autopsy Diagnosis. A Retrospective Study of 131 Newborns, Stillborns and Aborted Fetuses

Mariana COSTACHE, Monica CIRSTOIU, Andreea CONTOLENCO, Anca Mihaela LAZAROIU, Simion GEORGE, Maria SAJIN and Oana Maria PATRASCU

ABSTRACT

In recent years, the autopsy was considered necessary only in medico-legal cases, or when the clinician requires it to better understand the pathology and cause of death (with the deceased family’s consent). Although it has been shown in numerous studies that the autopsy and the postmortem histopathological examination are the only ones that can diagnose correctly and completely, the autopsy rate is declining. The primary motive of the family in consenting to a perinatal necropsy, may be to determine the cause of death of their child and to be aware of possible complications of their future pregnancy. This study shows the rate of concordance between clinical diagnosis and autopsy diagnosis, and the rate of concordance between macroscopic diagnosis and microscopic findings, pointing out once again the importance and the utility of the autopsy in medical practice.

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Clinical or Postmortem? The Importance of the Autopsy; a Retrospective Study

Mariana COSTACHE, Anca Mihaela LAZAROIU, Andreea CONTOLENCO, Diana COSTACHE, Simion GEORGE, Maria SAJIN and Oana Maria PATRASCU

ABSTRACT

Medicine is continually evolving; the new technologies of diagnosis and treatment continue to improve the life expectancy and lead to new information concerning various pathologies. The autopsy is viewed more and more as an ultimate branch of medicine and used only in extreme cases or for forensic purposes. Nevertheless, many studies, including this one, prove the utility and indispensability of the autopsies, without which a complete and accurate diagnosis cannot be made. Finally, the autopsy followed by histopathological examination of the tissues remains the ultimate and most important step for the apprehension of the diseases and for further evolution of medicine. This study reveals the correspondence rate between the clinical and the postmortem diagnosis, as well as between macroscopic and histopathological diagnosis.

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Oana Maria PATRASCU

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Articles signed on MÆDICA, JCM:

Expression of Bcl-2, Melan A and HMB-45 in Dysplastic Nevi

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